	US 12,146,823 B2
	Systems and methods for cell dissociation
Kam Lin Wong, Bedford, MA (US); and Melanie Scully, Beverly, MA (US)
Assigned to Thrive Bioscience, Inc., Beverly, MA (US)
Filed by Thrive Bioscience, Inc., Beverly, MA (US)
Filed on Mar. 20, 2023, as Appl. No. 18/186,827.
Application 18/186,827 is a continuation of application No. 16/614,538, granted, now 11,662,296, previously published as PCT/US2018/033413, filed on May 18, 2018.
Claims priority of provisional application 62/508,955, filed on May 19, 2017.
Prior Publication US 2023/0304918 A1, Sep. 28, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 15/1433 (2024.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); G01N 15/1434 (2024.01); G02B 21/32 (2006.01); G16B 50/30 (2019.01); G01N 15/14 (2006.01); G01N 21/64 (2006.01)

CPC G01N 15/1433 (2024.01) [C12M 41/46 (2013.01); C12M 41/48 (2013.01); G01N 15/1434 (2013.01); G02B 21/32 (2013.01); G16B 50/30 (2019.02); G01N 2015/1447 (2013.01); G01N 2015/1452 (2013.01); G01N 2015/1493 (2013.01); G01N 2015/1497 (2013.01); G01N 21/6458 (2013.01)]

21 Claims

1. A cell culture incubator comprising: an incubator cabinet configured to receive a cell culture vessel storing a plurality of cells being dissociated from at least one surface of the cell culture vessel by at least one cell dissociation agent; an imaging system configured to image the plurality of cells; and at least one controller coupled to the imaging system; the at least one controller coupled to memory containing instructions that when executed: control the imaging system to capture a sequence of images of at least some cells in the plurality of cells during dissociation; and use the sequence of images to identify when to neutralize the at least one cell dissociation agent, wherein capturing the sequence of images comprises capturing a first plurality of images of the at least some cells in a plurality of focal planes and subsequently capturing a second plurality of images of the at least some cells in a plurality of focal planes.