US 12,146,153 B2
Cellular microcompartment and preparation processes
Maxime Feyeux, Talence (FR); Kevin Alessandri, Bordeaux (FR); Pierre Nassoy, Bordeaux (FR); Laurent Cognet, Bordeaux (FR); Gaelle Recher, Talence (FR); and Erwan Bezard, Bordeaux (FR)
Assigned to UNIVERSITE DE BORDEAUX, Bordeaux (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE, Palaiseau (FR)
Filed by UNIVERSITE DE BORDEAUX, Bordeaux (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE, Palaiseau (FR)
Filed on Dec. 19, 2022, as Appl. No. 18/083,912.
Application 18/083,912 is a continuation of application No. 16/462,962, previously published as PCT/FR2017/053225, filed on Nov. 23, 2017.
Claims priority of application No. 1661377 (FR), filed on Nov. 23, 2016.
Prior Publication US 2023/0117999 A1, Apr. 20, 2023
Int. Cl. C12N 5/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12N 5/074 (2010.01)
CPC C12N 5/0075 (2013.01) [C12M 23/20 (2013.01); C12M 25/01 (2013.01); C12M 25/16 (2013.01); C12N 5/0012 (2013.01); C12N 5/0696 (2013.01); C12N 2501/727 (2013.01); C12N 2513/00 (2013.01); C12N 2533/74 (2013.01); C12N 2533/90 (2013.01); C12N 2535/00 (2013.01)] 8 Claims
 
1. A cell encapsulation device comprising at least:
syringe pumps on which are loaded solutions necessary for encapsulation, including a cell solution comprising pluripotent stem cells and a solution comprising alginate,
a microfluidic injector arranged to inject through a tip said solutions concentrically to form a jet that breaks into cell microcompartments, each cell microcompartment having an outer layer formed from the alginate solution and the outer layer enveloping a core which contains the cell solution,
a mass ring placed at a distance from the tip in the plane perpendicular to the axis of the jet leaving the microfluidic injector, the mass ring arranged to charge the alginate solution of the cell microcompartments with direct current (DC) when the cell microcompartments are passing through the mass ring; and
a calcium bath adapted to collect the cell microcompartments after the cell microcompartments have passed through the mass ring and to stiffen said outer layer to form a shell of said cell microcompartments.