US 12,146,146 B2
Method for producing trans-polyisoprenoid, vector, transgenic plant, method for producing pneumatic tire and method for producing rubber product
Yuko Sakurai, Kobe (JP); Haruhiko Yamaguchi, Kobe (JP); Yukino Inoue, Kobe (JP); Kazuhisa Fushihara, Kobe (JP); Seiji Takahashi, Sendai (JP); Satoshi Yamashita, Kanazawa (JP); and Toru Nakayama, Sendai (JP)
Assigned to SUMITOMO RUBBER INDUSTRIES, LTD., Kobe (JP); TOHOKU UNIVERSITY, Sendai (JP); and KANAZAWA UNIVERSITY, Kanazawa (JP)
Filed by SUMITOMO RUBBER INDUSTRIES, LTD., Kobe (JP); TOHOKU UNIVERSITY, Sendai (JP); and KANAZAWA UNIVERSITY, Kanazawa (JP)
Filed on Aug. 26, 2021, as Appl. No. 17/412,891.
Application 17/412,891 is a division of application No. 16/466,193, abandoned, previously published as PCT/JP2017/041732, filed on Nov. 21, 2017.
Claims priority of application No. 2016-248330 (JP), filed on Dec. 21, 2016.
Prior Publication US 2022/0002759 A1, Jan. 6, 2022
Int. Cl. C12N 15/82 (2006.01); A01H 6/00 (2018.01); B60C 1/00 (2006.01); C12P 5/02 (2006.01)
CPC C12N 15/8243 (2013.01) [A01H 6/00 (2018.05); B60C 1/00 (2013.01); C12P 5/02 (2013.01)] 7 Claims
 
1. A method for producing a trans-polyisoprenoid, the method comprising:
binding a protein expressed from a gene coding for a trans-prenyltransferase (tPT) family protein to rubber particles in vitro,
wherein said binding is that said protein is fully or partially incorporated into the rubber particle, or the protein is inserted into a membrane structure of the rubber particle, and
wherein the binding comprises performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a trans-prenyltransferase (tPT) family protein to bind the tPT family protein to the rubber particles.