US 12,145,978 B2
Composition comprising an antibody
Yoshiki Yamada, Tokyo (JP); Tatsuya Kiyasu, Tokyo (JP); Kazuyuki Tabata, Tokyo (JP); Akihiro Kuzumaki, Tokyo (JP); and Mai Yamashiro, Tokyo (JP)
Assigned to Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
Filed by Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
Filed on Apr. 16, 2021, as Appl. No. 17/233,008.
Application 17/233,008 is a division of application No. 15/589,000, filed on May 8, 2017, granted, now 11,136,375.
Application 15/589,000 is a division of application No. 12/734,094, granted, now 9,683,027, previously published as PCT/JP2008/068588, filed on Oct. 14, 2008.
Claims priority of application No. 2007-267384 (JP), filed on Oct. 15, 2007; and application No. 2008-103308 (JP), filed on Apr. 11, 2008.
Prior Publication US 2021/0300996 A1, Sep. 30, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/395 (2006.01); C07K 16/00 (2006.01)
CPC C07K 16/00 (2013.01) [C07K 2317/56 (2013.01); C12N 2510/02 (2013.01)] 11 Claims
 
1. A composition comprising an antibody or an antigen binding fragment thereof wherein the antibody is produced by a method comprising:
(a) culturing a cell to produce the antibody or an antigen binding fragment thereof, and
(b) purifying the antibody or an antigen binding fragment thereof to obtain the composition,
wherein the cell contains a larger number of copies of an exogenous DNA encoding the light chain or an antigen binding fragment thereof of the antibody than the number of copies contained in the cell of an exogenous DNA encoding the heavy chain or an antigen binding fragment thereof of the antibody, and
wherein the antibody or the antigen binding fragment thereof is present in a high concentration in said composition compared to a composition comprising the same antibody or the antigen binding fragment thereof by which the antibody is produced wherein the cell contains one copy of the DNA encoding heavy chain or the antigen binding fragment thereof and one copy of the DNA encoding the light chain or the antigen binding fragment thereof.