	US 12,145,976 B2
	Modified A1-A2 domains of non-natural NKG2D ligands that bind non-natural NKG2D receptors
Kaman C. Kim, San Bruno, CA (US); and Kyle E. Landgraf, Alameda, CA (US)
Assigned to XYPHOS BIOSCIENCES INC., South San Francisco, CA (US)
Filed by XYPHOS BIOSCIENCES INC., South San Francisco, CA (US)
Filed on Mar. 27, 2019, as Appl. No. 16/366,320.
Claims priority of provisional application 62/648,636, filed on Mar. 27, 2018.
Prior Publication US 2019/0300594 A1, Oct. 3, 2019
Int. Cl. C07K 14/74 (2006.01); A61K 35/17 (2015.01); A61P 35/00 (2006.01); C07K 14/705 (2006.01); C07K 16/28 (2006.01); C07K 16/46 (2006.01); C07K 19/00 (2006.01)

CPC C07K 14/7056 (2013.01) [A61K 35/17 (2013.01); A61P 35/00 (2018.01); C07K 14/70539 (2013.01); C07K 16/2833 (2013.01); C07K 16/2851 (2013.01)]

10 Claims

1. A non-natural, modified α1-α2 domain of a natural NKG2D ligand molecule, wherein said modified α1-α2 domain comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 4, and wherein in said amino acid sequence having at least 90% identity to SEQ ID NO: 4, the positions corresponding to positions 80, 154, 155, 157, 158 and 159 of SEQ ID NO: 4 are substituted as compared to SEQ ID NO: 4, and

wherein said amino acid sequence having at least 90% identity to SEQ ID NO: 4 has at least one of the following features:

the amino acid at the position corresponding to position 157 of SEQ ID NO: 4 is W; or

the amino acid at the position corresponding to position 158 of SEQ ID NO: 4 is Q, G, or S.