	US 12,145,965 B2
	Use of vitellogenin for defining and testing novel immunogens in insects
Huw Hughes, Los Angeles, CA (US)
Assigned to Dalan Animal Health, Inc., Athens, GA (US)
Appl. No. 18/043,723
Filed by Dalan Animal Health, Inc., Athens, GA (US)
PCT Filed Sep. 1, 2021, PCT No. PCT/US2021/048727 § 371(c)(1), (2) Date Mar. 1, 2023, PCT Pub. No. WO2022/051406, PCT Pub. Date Mar. 10, 2022.
Claims priority of provisional application 63/074,365, filed on Sep. 3, 2020.
Prior Publication US 2024/0018183 A1, Jan. 18, 2024
Int. Cl. C07K 1/16 (2006.01); C07K 14/435 (2006.01)

CPC C07K 1/16 (2013.01) [C07K 14/43572 (2013.01)]

11 Claims

1. A method to isolate a honey bee antigen comprising:

contacting recombinant vitellogenin (rVg) with a solid support to form rVg to the solid support;

contacting a solubilized inactivated honey bee pathogen with the rVg bound to the solid support; and

eluting the inactivated honey bee pathogen to isolate the honey bee antigen.