US 12,145,964 B2
Thiophosphorodichloridate reagents for chemoselective histidine bioconjugation
Christopher J. Chang, Berkeley, CA (US); and Shang Jia, Berkeley, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Mar. 20, 2021, as Appl. No. 17/207,609.
Application 17/207,609 is a continuation of application No. PCT/US2019/056018, filed on Oct. 13, 2019.
Claims priority of provisional application 62/749,670, filed on Oct. 23, 2018.
Prior Publication US 2021/0230215 A1, Jul. 29, 2021
Int. Cl. C07K 1/13 (2006.01); C07K 1/113 (2006.01); C07K 14/00 (2006.01)
CPC C07K 1/13 (2013.01) [C07K 1/113 (2013.01); C07K 14/00 (2013.01); C07K 2319/21 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method of labeling a protein comprising:
Reacting the protein with a thiophosphorodichloridate reagent under conditions to effect chemoselective histidine conjugation and form a resultant conjugate of the protein and the thiophosphorodichloridate reagent;
wherein the thiophosphorodichloridate reagent is selected form the group consisting of:

OG Complex Work Unit Chemistry