	US 12,144,910 B2
	Methods of coating antimicrobial peptides on the biomaterial and the biomaterial coated thereby
You-Di Liao, Taipei (TW); Dan-Wei Wang, New Taipei (TW); Eden Wu, Jiaoxi Township (TW); Shih-Han Wang, Taipei (TW); and Wen-Hung Tang, Taichung (TW)
Assigned to ACADEMIA SINICA, Taipei (TW)
Appl. No. 17/604,318
Filed by ACADEMIA SINICA, Taipei (TW)
PCT Filed Apr. 16, 2020, PCT No. PCT/US2020/028554 § 371(c)(1), (2) Date Oct. 15, 2021, PCT Pub. No. WO2020/214836, PCT Pub. Date Oct. 22, 2020.
Claims priority of provisional application 62/834,844, filed on Apr. 16, 2019.
Prior Publication US 2022/0226545 A1, Jul. 21, 2022
Int. Cl. A61L 27/54 (2006.01); A61L 29/16 (2006.01); A61L 31/16 (2006.01)

CPC A61L 27/54 (2013.01) [A61L 29/16 (2013.01); A61L 31/16 (2013.01); A61L 2300/252 (2013.01); A61L 2300/404 (2013.01); A61L 2300/606 (2013.01); A61L 2420/02 (2013.01)]

16 Claims

1. A method for coating a surface of a material, comprising

(i) dissolving one or more antimicrobial peptides (AMPs) in a buffer containing a lower concentration anionic surfactant than 0.002% (w/v) to form solution (a);

(ii) adding an amount of a buffer containing a higher concentration anionic surfactant than 0.02% (w/v) to the solution (a) to form solution (b), wherein the amount of the buffer containing the higher concentration anionic surfactant is equal to that of the buffer containing the lower concentration anionic surfactant; and

(iii) coating the solution (b) onto the surface of the material so that the AMPs attach onto the surface.