	US 12,144,871 B2
	Stable compositions of MRNA-loaded lipid nanoparticles and processes of making
Shrirang Karve, Lexington, MA (US); Frank DeRosa, Lexington, MA (US); Michael Heartlein, Lexington, MA (US); Natalia Vargas Montoya, Lexington, MA (US); Priyal Patel, Lexington, MA (US); and Ashish Sarode, Lexington, MA (US)
Assigned to TRANSLATE BIO, INC., Lexington, MA (US)
Filed by Translate Bio, Inc., Lexington, MA (US)
Filed on Jul. 23, 2020, as Appl. No. 16/936,909.
Claims priority of provisional application 62/877,597, filed on Jul. 23, 2019.
Prior Publication US 2021/0046192 A1, Feb. 18, 2021
Int. Cl. A61K 48/00 (2006.01); A61K 9/107 (2006.01); A61K 47/32 (2006.01); A61K 47/34 (2017.01)

CPC A61K 48/005 (2013.01) [A61K 9/1075 (2013.01); A61K 47/32 (2013.01); A61K 47/34 (2013.01); A61K 48/0041 (2013.01)]

16 Claims

1. A stable composition comprising lipid nanoparticles encapsulating messenger RNA (mRNA), wherein the mRNA encodes a protein or a peptide, wherein each of the lipid nanoparticles comprises one or more cationic lipids, one or more non-cationic lipids, an amphiphilic block co-polymer, and less than 0.5% of PEG-modified lipids or PEG, and wherein the lipid nanoparticles encapsulating mRNA comprise 0.5% or less of the amphiphilic block co-polymer.