	US 12,144,845 B2
	Use of lysin to restore/augment antibacterial activity in the presence of pulmonary surfactant of antibiotics inhibited thereby
Michael Wittekind, Bainbridge Island, WA (US); and Raymond Schuch, Mountain Lakes, NJ (US)
Assigned to Aurobac Therapeutics SAS, Lyons (FR)
Filed by AUROBAC THERAPEUTICS SAS, Lyons (FR)
Filed on May 20, 2022, as Appl. No. 17/749,252.
Application 17/749,252 is a division of application No. 15/760,416, granted, now 11,357,833, previously published as PCT/US2016/052348, filed on Sep. 16, 2016.
Claims priority of provisional application 62/220,212, filed on Sep. 17, 2015.
Claims priority of provisional application 62/247,619, filed on Oct. 28, 2015.
Prior Publication US 2022/0362352 A1, Nov. 17, 2022
Int. Cl. A61K 38/47 (2006.01); A61K 9/00 (2006.01); A61K 31/7036 (2006.01); A61K 38/12 (2006.01); A61K 38/16 (2006.01); A61K 38/46 (2006.01); A61K 45/06 (2006.01); A61P 31/04 (2006.01); C12N 9/36 (2006.01)

CPC A61K 38/47 (2013.01) [A61K 9/0019 (2013.01); A61K 9/0073 (2013.01); A61K 31/7036 (2013.01); A61K 38/12 (2013.01); A61K 38/162 (2013.01); A61K 38/46 (2013.01); A61K 45/06 (2013.01); A61P 31/04 (2018.01); C12N 9/2462 (2013.01); C12Y 302/01017 (2013.01); A61K 2121/00 (2013.01); Y02A 50/30 (2018.01)]

14 Claims

1. A method for treating a subject afflicted with a Gram-negative bacterial infection of an organ or tissue in which pulmonary surfactant is present, the method comprising regardless of order the following steps:

(a) administering to the subject an antibiotic having antibacterial activity against the Gram-negative bacteria responsible for the infection which activity is inhibited by the pulmonary surfactant;

(b) co-administering to the subject a lysin polypeptide,

wherein said antibiotic and said lysin polypeptide in combination are effective to kill the Gram-negative bacteria responsible for the infection and thereby treat the infection, and

wherein the lysin polypeptide comprises the sequences selected from SEQ ID NO: 6 (GN37); SEQ ID NO: 7 (GN2); SEQ ID NO: 9 (GN14); or SEQ ID NO: 10 (GN43).