System for the biocontrol of white spot syndrome virus (WSSV) in aquaculture
Richard T. Sayre, Los Alamos, NM (US); Tatiana Vinogradova-Shah, Los Alamos, NM (US); and Elena Sineva, Los Alamos, NM (US)
Assigned to Pebble Labs Inc., Los Alamos, NM (US)
Filed by Pebble Labs Inc., Los Alamos, NM (US)
Filed on Sep. 13, 2022, as Appl. No. 17/931,659.
Application 17/931,659 is a continuation of application No. 17/020,498, filed on Sep. 14, 2020, granted, now 11,446,339.
Application 17/020,498 is a continuation in part of application No. 16/588,498, filed on Sep. 30, 2019, granted, now 10,774,329, issued on Sep. 15, 2020.
Application 16/588,498 is a continuation in part of application No. PCT/US2018/025766, filed on Apr. 2, 2018.
Claims priority of provisional application 62/480,138, filed on Mar. 31, 2017.
Prior Publication US 2023/0047351 A1, Feb. 16, 2023
1. A composition for the biocontrol of white spot syndrome comprising a genetically modified bacteria expressing at least one heterologous nucleotide sequence having at least 95% homology with the nucleotide sequence according to SEQ ID NO. 15, encoding at least one inhibitory polynucleotide that downregulates expression of the vp19 gene of the white spot syndrome virus (WSSV) wherein said inhibitory polynucleotide comprises a first inhibitory RNA polynucleotide having at least 95% homology with the nucleotide sequence according to SEQ ID NO. 13, and a second complementary polynucleotide having at least 95% homology with the nucleotide sequence according to SEQ ID NO. 14, wherein said first and said second RNA polynucleotides form an inhibitory double stranded RNA molecule (dsRNA) that downregulates expression of the vp19 gene of WSSV.