	US 12,144,832 B1
	Plasma derived from human umbilical cord blood for the treatment of neurodegenerative disorders
Svitlana Garbuzova-Davis, Tampa, FL (US); Jared Carl Ehrhart, Tampa, FL (US); and Paul R. Sanberg, Spring Hill, FL (US)
Assigned to University of South Florida, Tampa, FL (US); and Saneron CCEL Therapeutics, Inc., Tampa, FL (US)
Filed by University of South Florida, Tampa, FL (US)
Filed on Apr. 14, 2023, as Appl. No. 18/134,722.
Application 18/134,722 is a division of application No. 16/655,506, filed on Oct. 17, 2019, granted, now 11,628,190.
Application 16/655,506 is a continuation in part of application No. 15/250,239, filed on Aug. 29, 2016, granted, now 11,007,230, issued on May 18, 2021.
Claims priority of provisional application 62/211,478, filed on Aug. 28, 2015.
Int. Cl. A61K 35/51 (2015.01); A61P 25/28 (2006.01); C12N 5/0775 (2010.01)

CPC A61K 35/51 (2013.01) [A61P 25/28 (2018.01); C12N 5/0665 (2013.01)]

3 Claims

1. A method of increasing umbilical cord blood cell (UCBC) viability and reducing apoptotic UCBC death comprising:

obtaining or having obtained cord blood from a subject;

diluting or having diluted the cord blood 1:1 with sterile phosphate buffered saline (PBS) in the absence of Mg₂⁺ and Ca₂⁺;

isolating or having isolated an amount of UCBCs from the diluted cord blood of the subject;

isolating or having isolated an amount of umbilical cord blood plasma from the diluted cord blood of the subject;

suspending the UCBCs in phenol-free RPMI-1640 media;

plating the UCBCs suspended in the phenol-free RPMI-1640 media;

adding about 10% of the umbilical cord blood plasma to the plated UCBCs suspended in the phenol-free RPMI-1640 media to form a culture media wherein the culture media consists of about 10% of the umbilical cord blood plasma and about 90% of the phenol-free RPMI-1640 media;

culturing the UCBCs in the culture media for about 5 days; and

removing the UCBCs from the culture media after about 5 days;

wherein the UCBC cell viability is at least 79% as compared to a control.