US 12,144,799 B2
Method for long-term storage of chlorophyll-containing extract
Byung Ju Ryu, Gwangju (KR); Jihwan Im, Gwangju (KR); Hyoungmin Yeo, Gwangju (KR); Myungeun Lee, Gwangju (KR); and Chulcuy Choi, Gwangju (KR)
Assigned to ABL CO., LTD, Gwangju (KR)
Appl. No. 17/290,839
Filed by ABL CO., LTD, Gwangju (KR)
PCT Filed Nov. 6, 2019, PCT No. PCT/KR2019/014999
§ 371(c)(1), (2) Date May 3, 2021,
PCT Pub. No. WO2020/096354, PCT Pub. Date May 14, 2020.
Claims priority of application No. 10-2018-0134973 (KR), filed on Nov. 6, 2018.
Prior Publication US 2021/0401801 A1, Dec. 30, 2021
Int. Cl. A23L 33/105 (2016.01); A23L 3/3463 (2006.01); A23L 33/12 (2016.01); A61K 31/409 (2006.01); A61K 36/00 (2006.01); A61K 47/12 (2006.01); A61K 47/44 (2017.01)
CPC A61K 31/409 (2013.01) [A23L 3/3463 (2013.01); A23L 33/105 (2016.08); A23L 33/12 (2016.08); A61K 36/00 (2013.01); A61K 47/12 (2013.01); A61K 47/44 (2013.01); A23V 2002/00 (2013.01); A61K 2236/37 (2013.01)] 11 Claims
 
1. A method for storing chlorophyll, the method comprising:
preparing chlorophyll to be stored; and
dissolving the chlorophyll in oil having an unsaturated lipid structure to allow the chlorophyll to be included in the oil,
wherein the preparation of the chlorophyll to be stored comprises:
pretreating a plant containing the chlorophyll and/or a microorganism containing the chlorophyll by mixing the plant and/or the microorganism with oil selected from the group consisting of soybean oil, canola oil, sunflower seed oil, macadamia oil, peanut oil, grapeseed oil, pumpkin seed oil, flaxseed oil, linseed oil, olive oil, corn oil, safflower oil, sesame oil, almond oil, peach seed oil, apricot seed oil, walnut oil, rapeseed oil, raspberry oil, bilberry seed oil, cranberry seed oil, pomegranate seed oil, sacha inchi oil, sea buckthorn seed oil, chia oil, perilla oil, and a mixture thereof;
removing an impurity by performing a first supercritical extraction on the plant and/or the microorganism with a solvent comprising carbon dioxide; and
preparing a chlorophyll extract by performing a second supercritical extraction on the plant and/or the microorganism with a solvent comprising carbon dioxide as a main solvent and ethanol as a co-solvent; and
wherein the dissolving of the chlorophyll in the oil comprises:
distilling the chlorophyll extract under reduced pressure and solidifying the chlorophyll to obtain the chlorophyll to be stored; and
dissolving the solidified chlorophyll in the oil selected from the group consisting of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), squalene, soybean oil, canola oil, sunflower seed oil, macadamia oil, peanut oil, grapeseed oil, pumpkin seed oil, flaxseed oil, linseed oil, olive oil, corn oil, safflower oil, sesame oil, pine-nut oil, conjugated linolenic acid, almond oil, peach seed oil, apricot seed oil, walnut oil, rapeseed oil, raspberry oil, bilberry seed oil, cranberry seed oil, pomegranate seed oil, sacha inchi oil, sea buckthorn oil, chia oil, perilla oil, diacylglycerol oil, plant-derived sources of omega 3, fermentation sources of eicosapentaenoic acid (EPA), fermentation sources of docosahexaenoic acid (DHA), fish oil, krill oil, sources of gamma linolenic acid (GLA) and/or stearidonic acid (SA), coconut oil and a combination thereof.