US 12,144,491 B2
Spectrally encoded imaging using band-shifting imaging probes
Zhiwen Liu, State College, PA (US); Jian Yang, State College, PA (US); Yizhu Chen, Fremont, CA (US); and Dingbowen Wang, State College, PA (US)
Assigned to The Penn State Research Foundation, University Park, PA (US)
Appl. No. 17/264,161
Filed by The Penn State Research Foundation, University Park, PA (US)
PCT Filed Jul. 30, 2019, PCT No. PCT/US2019/044144
§ 371(c)(1), (2) Date Jan. 28, 2021,
PCT Pub. No. WO2020/028367, PCT Pub. Date Feb. 6, 2020.
Claims priority of provisional application 62/712,647, filed on Jul. 31, 2018.
Prior Publication US 2021/0228066 A1, Jul. 29, 2021
Int. Cl. G01N 21/64 (2006.01); A61B 1/04 (2006.01); A61K 49/00 (2006.01); G01N 21/88 (2006.01)
CPC A61B 1/043 (2013.01) [A61K 49/0021 (2013.01); A61K 49/006 (2013.01); G01N 21/6428 (2013.01); G01N 21/6458 (2013.01); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6423 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/8893 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A spectrally encoded parallel fluorescence imaging system for imaging a sample, comprising:
a multi-wavelength excitation light source emitting excitation light with a plurality of different wavelengths;
optical components adapted for introducing spectral encoding in an axial direction such that the different wavelengths of the excitation light are focused at different axial positions in the sample and for introducing spectral coding in at least one lateral direction such that the different wavelengths of the excitation light are focused at different lateral positions in the sample thereby generating fluorescence signals emitted at the different axial and lateral positions, the axial direction coinciding or being parallel to an axis of the excitation light, the at least one lateral direction being perpendicular to the axis of the excitation light;
band-shifting florescence imaging probes adapted for introduction into the sample, the band-shifting florescence imaging probes comprising fluorophores exhibiting excitation-dependent emission bands, each wavelength of the excitation light corresponding to a band-shifted fluorescence spectrum, thereby causing a different band-shifted fluorescence spectrum emitted at each of the different axial and lateral positions and transferring the spectral encoding of the excitation light to the fluorescence emission signals at the different axial and lateral positions of the sample, the fluorescence signals emitted at different axial and lateral locations of the labeled sample being already distinguished in center wavelengths at the sample through use of the band-shifting florescence imaging probes; and
detectors operable to detect/collect different wavelengths for simultaneous detection/collection of the fluorescence signals emitted and already distinguished at the different axial and lateral positions of the sample, thereby enabling simultaneous recording of image signals of the sample at the different axial and lateral positions.