US 12,144,310 B2
Tobacco plant resistant to spotted wilt disease without linkage drag and method for breeding the same
Changjun Huang, Yunnan (CN); Yong Liu, Yunnan (CN); and Haiqin Yu, Yunnan (CN)
Assigned to Yunnan Academy of Tobacco Agricultural Sciences, Yunnan (CN)
Appl. No. 17/763,779
Filed by Yunnan Academy of Tobacco Agricultural Sciences, Yunnan (CN)
PCT Filed Nov. 8, 2021, PCT No. PCT/CN2021/129382
§ 371(c)(1), (2) Date Mar. 25, 2022,
PCT Pub. No. WO2022/262179, PCT Pub. Date Dec. 22, 2022.
Prior Publication US 2024/0049674 A1, Feb. 15, 2024
Int. Cl. A01H 6/82 (2018.01); A01H 1/00 (2006.01); A01H 1/02 (2006.01); A01H 1/04 (2006.01); C12Q 1/6895 (2018.01)
CPC A01H 6/823 (2018.05) [A01H 1/02 (2013.01); A01H 1/045 (2021.01); A01H 1/126 (2021.01); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01)] 10 Claims
 
1. A non-naturally occurring tobacco plant or germplasm with resistance to tobacco spotted wilt disease (TSWD) and without linkage drag, which is obtained by hybridization using tobacco variety ‘Polalta’ as TSWD resistance donor parent and a TSWD susceptible tobacco variety as recipient parent and recurrent parent, wherein said non-naturally occurring tobacco plant or germplasm comprises NaChr3_59M marker as set forth in SEQ ID NO: 5, and wherein said non-naturally occurring tobacco plant or germplasm does not comprise NaChr3_44.2M marker as set forth in SEQ ID NO: 1, NaChr3_54M marker as set forth in SEQ ID NO: 2, NaChr3_57M marker as set forth in SEQ ID NO: 3, NaChr3 58M marker as set forth in SEQ ID NO: 4, NaChr3_64.6M marker as set forth in SEQ ID NO: 8, NaChr4_2M marker as set forth in SEQ ID NO: 10 and NaChr4 8M marker as set forth in SEQ ID NO: 11.