	US 12,474,355 B2
	Combinations of biomarkers for methods for detecting trisomy 21
Carl Philip Weiner, Mission Hills, KS (US); and Yafeng Dong, Overland Park, KS (US)
Assigned to ROSETTA SIGNALING LABORATORIES, LLC
Filed by Carl Philip Weiner, Mission Hills, KS (US); and Yafeng Dong, Overland Park, KS (US)
Filed on Mar. 16, 2021, as Appl. No. 17/203,534.
Application 17/203,534 is a continuation in part of application No. 14/361,895, abandoned, previously published as PCT/US2012/067328, filed on Nov. 30, 2012.
Claims priority of provisional application 61/565,761, filed on Dec. 1, 2011.
Prior Publication US 2021/0199673 A1, Jul. 1, 2021
Int. Cl. G01N 33/53 (2006.01); G01N 33/68 (2006.01)

CPC G01N 33/6896 (2013.01) [G01N 33/5308 (2013.01); G01N 2800/387 (2013.01)]

12 Claims

1. A method of detecting a combination of ribonucleic acid (RNA) biomarkers, the method comprising:

obtaining a plasma sample from a human subject, wherein the human subject is a pregnant female;

obtaining cell free nucleic acids from the plasma sample, wherein the cell free nucleic acids are cell free ribonucleic acids (RNA);

detecting in the cell free RNA the presence of the combination of RNA biomarkers,

wherein the detecting comprises:

contacting the cell free RNA with a combination of primers or probes that are complementary to the combination of RNA biomarkers, and

detecting hybridization between the primers or probes and the combination of RNA biomarkers,

wherein the combination of RNA biomarkers used in the method consists of:

ATP5O, ICOSLG, DOP1B, PKNOX1, COL6A1, and GART.