	US 12,474,331 B2
	Method of isolating exosomes
Stephanie Duguez, Londonderry (GB); William Duddy, Londonderry (GB); and Owen Connolly, Londonberry (GB)
Assigned to INNOVATION ULSTER LIMITED UNIVERSITY OF ULSTER, TEIC BUILDING, JORDANSTOWN CAMPUS NEWTOWNABBEY, County Antrim Northern Ireland (IE)
Appl. No. 17/781,498
Filed by UNIVERSITY OF ULSTER, Londonderry (GB)
PCT Filed Dec. 4, 2020, PCT No. PCT/EP2020/084640 § 371(c)(1), (2) Date Jun. 1, 2022, PCT Pub. No. WO2021/110920, PCT Pub. Date Jun. 10, 2021.
Claims priority of application No. 1917698 (GB), filed on Dec. 4, 2019.
Prior Publication US 2023/0003726 A1, Jan. 5, 2023
Int. Cl. G01N 33/543 (2006.01); G01N 33/50 (2006.01)

CPC G01N 33/54326 (2013.01) [G01N 33/5002 (2013.01); G01N 2400/02 (2013.01)]

11 Claims

1. A method of isolating exosomes, the method comprising the steps of:

(a) providing a binding partner that binds to a dystroglycan (DAG) polypeptide present on the cell surface of exosomes;

(b) providing a sample that includes exosomes;

(c) contacting said binding partner with the sample under conditions that allow the binding partner to bind to the DAG polypeptide present on the cell surface of the exosomes;

(d) isolating the binding partner after step (c) and

(e) eluting the exosomes from the binding partner of step (d).