US 12,474,330 B2
Detection units and methods for detecting a target analyte
Alfredo A. Celedon, Columbia, MD (US); and Joseph Patrick Russell, Odenton, MD (US)
Assigned to Scanogen Inc., Baltimore, MD (US)
Filed by Scanogen Inc., Baltimore, MD (US)
Filed on May 23, 2022, as Appl. No. 17/750,987.
Application 17/750,987 is a continuation of application No. 16/420,231, filed on May 23, 2019, granted, now 11,371,986.
Claims priority of provisional application 62/676,439, filed on May 25, 2018.
Prior Publication US 2022/0291212 A1, Sep. 15, 2022
Int. Cl. G01N 33/543 (2006.01)
CPC G01N 33/54306 (2013.01) [G01N 33/54313 (2013.01); G01N 33/54393 (2013.01)] 32 Claims
 
1. A method of detecting two or more different target analytes in a sample, the method comprising:
a) providing at least one detectable piece coupled to a solid support via a complex formed by a target analyte and a first probe and a second probe, wherein:
i) the first probe is coupled to the detectable piece and bound to the target analyte if present, and
ii) the second probe is coupled to the solid support and bound to the target analyte if present, so that only if the target analyte is present in the sample, the detectable piece is directly or indirectly coupled to the solid support at an initial location via the complex, wherein the complex comprises an elongated region that is at least 100 nanometers in length;
b) either applying a force to the detectable piece and measuring the displacement of the detectable piece or measuring the amount of Brownian motion of the detectable piece;
c) exposing the complex to a disruptor that is capable of uncoupling the detectable piece from the solid support;
d) optionally applying a further force to the detectable piece; and
e) detecting if the detectable piece was uncoupled from the solid support by the disruptor; wherein presence of the target analyte in the sample is indicated by the detectable pieces:
i) suffering the displacement or the amount of Brownian motion within a pre-determined range and
ii) uncoupling from the solid support by the disruptor,
wherein a different target analyte is detected by using a first probe specific to the different target analyte and a second probe specific to the different target analyte, wherein the different target analyte, the first probe specific to the different target analyte, and the second probe specific to the different target analyte form a further complex, and wherein the disruptor optionally disrupts the further complex.