US 12,474,322 B2
Methods and compositions for reducing antibiotic administration to farm animals
Joy Parr Drach, Pontiac, IL (US); Mitchell Hockett, Raleigh, NC (US); Stefano Bresolin, Garner, NC (US); Erik J Jensen, Hillsborough, NC (US); Tobias M. Heineck, Durham, NC (US); Jasper N. Pollard, Durham, NC (US); Deborah Asion, Durham, NC (US); Rodolfo R. Rodriguez, Cary, NC (US); David A. Calderwood, Chapel Hill, NC (US); Norah Gerow Bate, Raleigh, NC (US); John Richard Sink, Raleigh, NC (US); and Jorge Carlos Correa, Raleigh, NC (US)
Assigned to Advanced Animal Diagnostics, Inc., Morrisville, NC (US)
Filed by Advanced Animal Diagnostics, Inc., Morrisville, NC (US)
Filed on Feb. 12, 2021, as Appl. No. 17/175,114.
Application 17/175,114 is a continuation of application No. 15/466,814, filed on Mar. 22, 2017, abandoned.
Claims priority of provisional application 62/311,706, filed on Mar. 22, 2016.
Prior Publication US 2021/0270803 A1, Sep. 2, 2021
Int. Cl. G01N 33/49 (2006.01)
CPC G01N 33/492 (2013.01) 23 Claims
 
1. A method of identifying and managing an animal or group of animals at a feedlot or confined animal feeding operation for which antibiotic treatment, anti-infective treatment, hospitalizing, quarantining, periodic weight recording, and/or culling is appropriate, comprising:
a) collecting a blood sample from said animal or each animal in said group of animals;
b) performing a leukocyte differential cell count on said sample;
c) comparing said leukocyte differential cell count of (b) with an index of infection for eosinophils in combination with at least two cell types selected from neutrophils, non-segmented neutrophils, immature non-segmented neutrophils including band neutrophils, lymphocytes, monocytes, and basophils;
d) determining need for treatment and/or effect on performance on the basis of said comparing step; and
e) initiating antibiotic treatment, anti-infective treatment, hospitalizing, quarantining, periodic weight recording, and/or culling of said animal or group of animals on the basis of said determining step;
wherein steps a)-d) are carried out in less than 3 minutes for each animal.