US 12,474,311 B2
Method for establishing fingerprint of traditional chinese medicine compound or its preparation with improving cognition
Shengcan Zou, Qingdao (CN); Shanglong Wang, Qingdao (CN); Xin Li, Qingdao (CN); Lei Zong, Qingdao (CN); Jiancheng Zong, Qingdao (CN); and Zengliang Zhang, Qingdao (CN)
Assigned to QINGDAO CHENLAND HEALTH INDUSTRY GROUP CO., LTD., Qingdao (CN); and CHENLAND NUTRITIONALS, INC., Irvine, CA (US)
Filed by Chenland Nutritionals, Inc., Irvine, CA (US); and Qingdao Chenland Biological Technology Co., ltd., Qingdao (CN)
Filed on May 12, 2022, as Appl. No. 17/743,414.
Application 17/743,414 is a continuation of application No. PCT/CN2019/126537, filed on Dec. 19, 2019.
Claims priority of application No. 201911112565.8 (CN), filed on Nov. 14, 2019.
Prior Publication US 2022/0326200 A1, Oct. 13, 2022
Int. Cl. G01N 30/86 (2006.01); A61K 35/00 (2006.01); G01N 30/14 (2006.01); G01N 30/06 (2006.01)
CPC G01N 30/8686 (2013.01) [A61K 35/00 (2013.01); G01N 30/14 (2013.01); G01N 2030/062 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A method for establishing fingerprint of traditional Chinese medicine compound or its preparation with improving cognition, wherein the traditional Chinese medicine compound is made from following raw materials: 1-20 parts by weight of Gastrodiae Rhizoma, 1-15 parts by weight of Polygala tenuifolia, 1-30 parts by weight of Acorus tatarinowii, 0.1-10 parts by weight of Cistanche deserticola Ma, 0.1-10 parts by weight of Rehmanniae Radix Praeparata, and 0.01-1 parts by weight of curcumin;
a chromatogram of a test sample solution is established by high performance liquid chromatography, chromatographic conditions are as follows: a chromatographic column is octadecyl silane bonded silica gel chromatographic column; a column temperature is 25˜35° C.; a flow rate is 0.9˜1.1 ml/min; detection wavelengths are 210˜230 nm and 310˜330 nm respectively; a mobile phase A is acetonitrile and a mobile phase B is water; and a gradient elution procedure is as follows:
 
 
 
 Time/min Mobile phase A/% Mobile phase B/%
 
 
 
 
   0.00~5.00 5 95
 
   5.00~8.00 5→8 95→92
 
   8.00~11.00 8 92
 
  11.00~17.00 8→13 92→87
 
  17.00~23.00 13 87
 
  23.00~26.00 13→17 87→83
 
  26.00~30.00 17 83
 
  30.00~31.00 17→21.5 83→78.5
 
  31.00~73.00 21.5→31 78.5→69
 
  73.00~93.00 31→50 69→50
 
  93.00~103.00 50→60 50→40
 
 103.00~108 60→70 40→30
 
 108.00~110.00 70→90 30→10
 
 110.00~120.00 90 10
 
 
wherein a preparation method of the test sample solution is as follows: taking the traditional Chinese medicine compound or its preparation, mixing it with an acetonitrile aqueous solution, wherein a ratio of the traditional Chinese medicine compound or its preparation to the acetonitrile aqueous solution is (0.5˜1.5):50 in g/ml; performing an ultrasonic treatment; filtering and taking a subsequent filtrate.