US 12,474,297 B2
Method for trapping microparticle and microparticle trapping device
Yuji Kimura, Hamamatsu (JP); Kazuya Suzuki, Hamamatsu (JP); Ryo Makino, Hamamatsu (JP); and Hiroyasu Itoh, Hamamatsu (JP)
Assigned to HAMAMATSU PHOTONICS K.K., Hamamatsu (JP)
Filed by HAMAMATSU PHOTONICS K.K., Hamamatsu (JP)
Filed on Dec. 10, 2021, as Appl. No. 17/547,377.
Claims priority of application No. 2020-206935 (JP), filed on Dec. 14, 2020.
Prior Publication US 2022/0187240 A1, Jun. 16, 2022
Int. Cl. B03C 5/00 (2006.01); B03C 5/02 (2006.01); G01N 21/64 (2006.01); G01N 27/447 (2006.01)
CPC G01N 27/44721 (2013.01) [B03C 5/005 (2013.01); B03C 5/022 (2013.01); G01N 21/64 (2013.01); G01N 2021/6495 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method for trapping a microparticle through dielectrophoresis, the method comprising:
preparing a fluid channel, first and second electrodes configured to generate an electric field in the fluid channel, and an electrical insulator including two partition walls intersecting each other, each of the two partition walls including an opening formed in the fluid channel between the first electrode and the second electrode;
injecting a fluid including the microparticle into the fluid channel; and
applying a voltage to the first and second electrodes so that an inhomogeneous electric field is made through the opening between the first and second electrodes in the fluid channel,
wherein the first electrode includes a first pair of electrodes opposing each other in a first direction, the second electrode includes a second pair of electrodes opposing each other in a second direction intersecting the first direction,
the preparing a fluid channel, first and second electrodes comprises preparing a housing for the fluid channel, the housing including a base and a wall defining the fluid channel with the base, the first and second electrodes being disposed on the base, the electrical insulator being disposed on the wall,
preparing the two partition walls to form four divisions in the fluid channel, and four electrodes including the first pair of electrodes and the second pair of electrodes, each of the four electrodes being disposed at one of the four divisions, and
the injecting a fluid comprises injecting the fluid including the microparticle into the fluid channel so that the microparticle comes in contact with the four electrodes, and disposing the wall on the base so that the opening is positioned in the fluid including the microparticle.