US 12,474,240 B2
Stabilized two-part hematoxylin solution utilizing pH adjustment
Edward E. Durrant, Oro Valley, AZ (US); and Christopher A. Koerber, Tucson, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Dec. 2, 2021, as Appl. No. 17/540,450.
Application 17/540,450 is a division of application No. 16/653,440, filed on Oct. 15, 2019, abandoned.
Application 16/653,440 is a continuation of application No. PCT/EP2018/061757, filed on May 8, 2018.
Claims priority of provisional application 62/504,066, filed on May 10, 2017.
Prior Publication US 2022/0090993 A1, Mar. 24, 2022
Int. Cl. G01N 1/30 (2006.01); C09B 65/00 (2006.01); C09B 67/10 (2006.01); C09B 67/14 (2006.01); C01D 1/00 (2006.01)
CPC G01N 1/30 (2013.01) [C09B 65/00 (2013.01); C09B 67/0014 (2013.01); C09B 67/0017 (2013.01); C01D 1/00 (2013.01); G01N 2001/302 (2013.01); Y10S 436/826 (2013.01)] 17 Claims
 
1. A method of staining a biological sample comprising: (a) providing a first reservoir comprising a stabilized hematoxylin formulation having a pH of less than 2.4, wherein the stabilized hematoxylin formulation comprises a hematoxylin dye and an acid in an amount ranging from 0.2% to 4% by total volume of the stabilized hematoxylin formulation; (b) mixing an aliquot of the stabilized hematoxylin formulation from the first reservoir with a base to increase the pH of the aliquot of the obtained stabilized hematoxylin formulation to form a hematoxylin staining solution having a pH ranging from 2.4 to 2.6, wherein the base is provided in a second reservoir, and wherein the hematoxylin staining solution is formed in a third reservoir; and (c) after forming the hematoxylin staining solution having the pH ranging from 2.4 to 2.6 in the third reservoir, dispensing with an automated staining apparatus the formed hematoxylin staining solution having the pH ranging from 2.4 to 2.6 to the biological sample.