US 12,473,601 B2
Methods for species-level resolution of microorganisms
Ryan Kemp, Irvine, CA (US); Mike Weinstein, Irvine, CA (US); and Shuiquan Tang, Irvine, CA (US)
Assigned to ZYMO RESEARCH CORPORATION, Irvine, CA (US)
Filed by ZYMO RESEARCH CORPORATION, Irvine, CA (US)
Filed on Jul. 5, 2023, as Appl. No. 18/347,064.
Application 18/347,064 is a continuation of application No. 16/933,920, filed on Jul. 20, 2020, granted, now 11,807,909.
Claims priority of provisional application 62/899,531, filed on Sep. 12, 2019.
Prior Publication US 2024/0209458 A1, Jun. 27, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/689 (2018.01)
CPC C12Q 1/689 (2013.01) [C12Q 1/6869 (2013.01)] 23 Claims
 
1. A method for the identification and/or quantification of one or more microbes comprising:
(a) extracting DNA from a microbial sample;
(b) performing 16S ribosomal RNA gene-targeted sequencing with a means for amplifying the V1-V3 region of the 16S ribosomal RNA gene and/or means for amplifying the V3-V4 region of the 16S ribosomal RNA gene, thereby obtaining DNA sequences; and
(c) analyzing the DNA sequences to identify the species of the one or more microbes.