	US 12,473,583 B2
	Use of guar gum, fluorescence-enhanced gold nanocluster, method for detecting alpha-glucosidase, and method for screening alpha-glucosidase inhibitor
Zhenzhen Huang, Changchun (CN); Xiaoyu Geng, Changchun (CN); Ruisong Xue, Changchun (CN); Shiyong Teng, Changchun (CN); Jinshuo Li, Changchun (CN); Yanmei Liu, Changchun (CN); and Wensheng Yang, Changchun (CN)
Assigned to Jilin University, Changchun (CN)
Filed by Jilin University, Changchun (CN)
Filed on Aug. 19, 2022, as Appl. No. 17/820,891.
Claims priority of application No. 202210655257.5 (CN), filed on Jun. 10, 2022.
Prior Publication US 2023/0399675 A1, Dec. 14, 2023
Int. Cl. C12Q 1/40 (2006.01); G01N 21/64 (2006.01)

CPC C12Q 1/40 (2013.01) [C12Y 302/0102 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01)]

8 Claims

1. A method for detecting an α-glucosidase (α-Glu) activity, comprising the following steps:

providing a fluorescence-enhanced gold nanocluster-Fe³⁺ mixed dispersion, wherein the fluorescence-enhanced gold nanocluster-Fe³⁺ mixed dispersion comprises a fluorescence-enhanced gold nanocluster prepared from gold nanoclusters, guar gum, and water as raw materials;

mixing an α-Glu solution to be tested, an L-ascorbic acid-2-O-α-D-glucopyranoside (AAG) solution, and a phosphate-buffered saline (PBS), and conducting first incubation to obtain a first incubation solution;

mixing the first incubation solution with the fluorescence-enhanced gold nanocluster-Fe³⁺ mixed dispersion, and conducting second incubation to obtain a second incubation solution; and

subjecting the second incubation solution to fluorescence detection, and obtaining an activity of the α-Glu to be tested according to a working curve of the α-Glu activity and a fluorescence emission intensity obtained by the fluorescence detection, wherein the fluorescence detection is conducted at a wavelength of 610 nm.