US 12,473,521 B2
Three-dimensional cell culture container
Jeong Ah Kim, Daejeon (KR); Young Hye Kim, Cheongju-si (KR); Yeong Jun Yu, Cheongju-si (KR); Seok Chung, Seoul (KR); and Ji Hun Yang, Incheon (KR)
Assigned to KOREA BASIC SCIENCE INSTITUTE, Daejeon (KR); and KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, Seoul (KR)
Appl. No. 16/645,539
Filed by KOREA BASIC SCIENCE INSTITUTE, Daejeon (KR); and KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, Seoul (KR)
PCT Filed Dec. 20, 2017, PCT No. PCT/KR2017/015160
§ 371(c)(1), (2) Date Mar. 9, 2020,
PCT Pub. No. WO2019/050101, PCT Pub. Date Mar. 14, 2019.
Claims priority of application No. 10-2017-0114829 (KR), filed on Sep. 7, 2017.
Prior Publication US 2020/0283711 A1, Sep. 10, 2020
Int. Cl. C12M 1/32 (2006.01); C12M 3/00 (2006.01); C12N 5/0735 (2010.01); C12N 5/074 (2010.01); C12N 5/00 (2006.01); C12N 5/0793 (2010.01)
CPC C12M 23/12 (2013.01) [C12M 21/08 (2013.01); C12N 5/0606 (2013.01); C12N 5/0607 (2013.01); C12N 5/0062 (2013.01); C12N 5/0619 (2013.01); C12N 2513/00 (2013.01); C12N 2535/00 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A cell culture container for three-dimensional cell cultures including:
a lower plate;
a barrier part comprising pillars provided on a top side of the lower plate;
an upper plate which finishes an opened end portion of the barrier part; and
one or more cell sample injection guide parts, provided in one side of the upper plate, oriented at a predetermined height relative to a culture portion of the lower plate enclosed by the barrier part, and configured to guide injection of a gel-type sample of a predetermined amount and at the predetermined height, to the culture portion,
wherein the pillars are radially arranged between the upper plate and the lower plate such that the barrier part is formed in a circular shape,
wherein the pillars of the barrier part have a gap of 200 μm to 2,000 μm therebetween,
wherein the radial arrangement and the gap of the barrier part are configured to maintain a surface tension of the gel-type sample and prevent elution of the gel-type sample, of the predetermined amount and at the predetermined height from the culture portion,
wherein the barrier part is configured to permit material exchange between the gel-type sample and a peripheral medium,
wherein the predetermined height is above the lower plate and below the upper plate,
wherein the barrier part extends perpendicularly from the top side of the lower plate to a bottom side of the upper plate to thereby separate the upper plate from the lower plate, which are substantially parallel to each other, by a gap therebetween of 0.05 mm to 5 mm,
wherein the upper plate does not extend beyond the barrier part,
wherein the pillars each have a diameter or width of 300 μm to 2,000 μm and
wherein the circular shape of the barrier part has an inner diameter of 2 mm to 10 mm.