US 12,473,433 B2
Spacing linker group design for brightness enhancement in dimeric or polymeric dyes
Hesham Sherif, New York, NY (US)
Assigned to Sony Group Corporation, Tokyo (JP)
Filed by SONY GROUP CORPORATION, Tokyo (JP)
Filed on Feb. 9, 2024, as Appl. No. 18/438,105.
Application 18/438,105 is a continuation of application No. 18/256,125, previously published as PCT/US2021/062235, filed on Dec. 7, 2021.
Claims priority of provisional application 63/219,706, filed on Jul. 8, 2021.
Claims priority of provisional application 63/122,285, filed on Dec. 7, 2020.
Prior Publication US 2024/0287313 A1, Aug. 29, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C09B 69/10 (2006.01); C09B 69/00 (2006.01); G01N 1/30 (2006.01); G01N 33/58 (2006.01)
CPC C09B 69/101 (2013.01) [C09B 69/00 (2013.01); G01N 1/30 (2013.01); G01N 33/582 (2013.01)] 15 Claims
 
1. A compound having the following structure (Ib):

OG Complex Work Unit Chemistry
or a stereoisomer, salt or tautomer thereof, wherein:
M1 is a FRET acceptor;
M2 is a FRET donor;
L1 is at each occurrence, independently either i) an alkylene linker; or ii) a, heteroalkylene, heteroalkenylene, heteroalkynylene or heteroatomic linker including at least one nitrogen atom;
L2 and L3 are, at each occurrence, independently an alkylene or heteroalkylene linker;
R1 is, at each occurrence, H;
R2 and R3, each independently, comprises a deoxythymidine (dT) group or are Q, or a protected form thereof, or L′;
R4 is, at each occurrence, independently OH, O−, or ORd;
R5 is, at each occurrence, oxo;
Rd is a counter ion;
Q is, at each occurrence, independently a moiety comprising a reactive group, or protected form thereof, capable of forming a covalent bond with an analyte molecule, a targeting moiety, or a complementary reactive group Q′;
L1 is a linker comprising a covalent bond to Q;
m is, at each occurrence, independently an integer of one or greater;
n is an integer of one or greater;
z is an integer from 15 to 30;
R7, R8, R9 and R10 are, at each occurrence, H; and
y1 and y2 are, at each occurrence, independently an integer from 3 to 6.