US 12,473,349 B2
PD-1 variants having increased PD-L1 affinity
Sang-Taek Jung, Seoul (KR); and Ji-Yeon Ha, Seoul (KR)
Assigned to KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, Seoul (KR)
Appl. No. 17/923,472
Filed by KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, Seoul (KR)
PCT Filed May 6, 2021, PCT No. PCT/KR2021/005657
§ 371(c)(1), (2) Date Nov. 8, 2022,
PCT Pub. No. WO2021/225378, PCT Pub. Date Nov. 11, 2021.
Claims priority of application No. 10-2020-0053981 (KR), filed on May 6, 2020; application No. 10-2020-0098405 (KR), filed on Aug. 6, 2020; and application No. 10-2020-0129925 (KR), filed on Oct. 8, 2020.
Prior Publication US 2023/0183318 A1, Jun. 15, 2023
Int. Cl. C07K 14/705 (2006.01); C12N 15/63 (2006.01); G01N 33/574 (2006.01); G01N 33/68 (2006.01)
CPC C07K 14/70596 (2013.01) [C12N 15/63 (2013.01); G01N 33/574 (2013.01); G01N 33/68 (2013.01)] 23 Claims
 
1. A programmed cell death protein-1 (PD-1) variant having increased binding ability to programmed death-ligand 1 (PD-L1), the PD-1 variant comprising amino acid substitutions of F13I, M46I, C69T and G100V in amino acids of wild-type PD-1 of SEQ ID NO:
1.