US 12,473,344 B2
Chimeric antigen receptor polypeptides and methods of using same
Crystal Mackall, Stanford, CA (US); Michael Lin, Stanford, CA (US); Robbie Majzner, Palo Alto, CA (US); Hokyung Chung, San Diego, CA (US); and Louai Labanieh, Stanford, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Appl. No. 17/254,618
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
PCT Filed Jul. 3, 2019, PCT No. PCT/US2019/040572
§ 371(c)(1), (2) Date Dec. 21, 2020,
PCT Pub. No. WO2020/010239, PCT Pub. Date Jan. 9, 2020.
Claims priority of provisional application 62/694,830, filed on Jul. 6, 2018.
Prior Publication US 2021/0171602 A1, Jun. 10, 2021
Int. Cl. C07K 14/705 (2006.01); A61K 31/426 (2006.01); A61K 31/498 (2006.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); C07K 14/725 (2006.01); C07K 16/28 (2006.01); C12N 9/50 (2006.01)
CPC C07K 14/7051 (2013.01) [A61K 31/426 (2013.01); A61K 31/498 (2013.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/421 (2025.01); C07K 16/28 (2013.01); C12N 9/506 (2013.01); A61K 2239/31 (2023.05); A61K 2239/38 (2023.05); A61K 2239/47 (2023.05); A61K 2239/48 (2023.05); C07K 2317/622 (2013.01); C07K 2319/50 (2013.01)] 10 Claims
 
1. A T cell expressing a polypeptide comprising from N-terminus to C-terminus:
a chimeric antigen receptor (CAR) comprising a single chain variable fragment (scFv);
a hepatitis C virus (HCV) nonstructural protein 3 (NS3) protease; and
a degron comprising an amino acid sequence having 90% or greater identity to the amino acid sequence of SEQ ID NO:1,
wherein the polypeptide further comprises a cleavage site for the protease disposed between the CAR and the protease.