US 12,472,499 B2
Particle separator system, materials, and methods of use
Geoffrey Facer, Redwood City, CA (US); Theodorus Evan de Groot, Oakland, CA (US); Kevin Travers, Menlo Park, CA (US); and Lesley Suzanne Weaver, Palo Alto, CA (US)
Assigned to LEVITASBIO, INC., Menlo Park, CA (US)
Filed by LEVITASBIO, INC., Menlo Park, CA (US)
Filed on Sep. 29, 2021, as Appl. No. 17/449,438.
Claims priority of provisional application 63/086,033, filed on Sep. 30, 2020.
Prior Publication US 2022/0097062 A1, Mar. 31, 2022
Int. Cl. B01L 3/00 (2006.01); G01N 15/06 (2024.01); G01N 15/075 (2024.01)
CPC B01L 3/502753 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502746 (2013.01); B01L 3/502761 (2013.01); G01N 15/0656 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/043 (2013.01); G01N 15/075 (2024.01)] 25 Claims
OG exemplary drawing
 
1. A method for separation of a mixture of live cells and dead cells comprising:
providing a flowcell cartridge comprising a processing channel, and a plurality of outlet channels, wherein said processing channel has a top vertical surface and a bottom vertical surface;
flowing a sample solution comprising live cells and dead cells and a paramagnetic compound into the processing channel;
placing the flowcell cartridge in a magnetic field substantially aligned parallel to the processing channel;
maintaining the processing channel and the sample solution contained therein entirely within the magnetic field in a stopped flow condition for a period of time sufficient to separate live cells and dead cells by a vertical distance within the processing channel; and
simultaneously withdrawing a sample fraction enriched with live cells and a sample fraction enriched with dead cells into the outlet channels;
wherein the paramagnetic compound is dissolved in solution and is present in an amount of from about 50 mM to about 200 mM.