	US 12,472,286 B2
	Compositions comprising textured patterns and methods of using the same
Wolfgang Losert, Bethesda, MD (US); John T. Fourkas, Bethesda, MD (US); Meghan Driscoll, Berwyn Heights, MD (US); Floyd Bates, II, Upper Marlboro, MD (US); Xiaoyu Sun, Laurel, MD (US); Can Guven, Hilsboro, OR (US); and Joshua Parker, Washington, DC (US)
Assigned to University of Maryland, College Park, MD (US)
Filed by University of Maryland, College Park, College Park, MD (US)
Filed on Nov. 28, 2022, as Appl. No. 18/059,288.
Application 18/059,288 is a division of application No. 15/420,016, filed on Jan. 30, 2017, abandoned.
Claims priority of provisional application 62/288,134, filed on Jan. 28, 2016.
Prior Publication US 2023/0181799 A1, Jun. 15, 2023
Int. Cl. A61L 27/50 (2006.01); A61F 13/02 (2006.01)

CPC A61L 27/50 (2013.01) [A61F 13/0243 (2013.01)]

19 Claims

1. A method of guiding unidirectional movement of a first cell in a sample comprising different cell types, the method comprising:

exposing the sample to a surface comprising a three-dimensional pattern of one or a plurality of asymmetric topographical elements for a time period sufficient to bias actin polymerization within the first cell;

biasing movement of the first cell in a first direction along the asymmetric topographical elements; and

biasing movement of a second cell in a second direction along the asymmetric topographical elements, wherein the first and the second cells are different cell types and the first and second directions are different;

wherein the topographical elements are from about 0.001 to about 100 microns in height or width or depth and aligned along at least one or a plurality of straight, curved or circumferential paths on the surface.