| CPC A61K 39/215 (2013.01) [A61P 31/14 (2018.01); A61K 2039/545 (2013.01); A61K 2039/55577 (2013.01); C12N 2770/20034 (2013.01)] | 12 Claims |

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1. A method of stimulating an immune response against SARS-CoV-2 in a human comprising administering an immunogenic composition to the human, wherein the immunogenic composition compromises:
(i) a nanoparticle comprising coronavirus S (CoV S) glycoprotein trimers and a non-ionic detergent core,
wherein individual CoV S glycoproteins of the glycoprotein trimers comprise a sequence that is at least 95% identical to SEQ ID NO: 2;
wherein amino acids 669-672 of the CoV S individual glycoproteins are QQAQ (SEQ ID NO: 7) and amino acids 973 and 974 are proline,
wherein said amino acids are numbered with respect to SEQ ID NO: 2; and
(ii) a saponin adjuvant;
wherein the immunogenic composition comprises from about 3 μg to about 25 μg of the CoV S glycoprotein, and
wherein the immunogenic composition comprises from about 1 μg to about 100 μg of adjuvant.
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