	US 12,472,242 B2
	Proteolytic enzyme composition
Anupam Trehan, Gurgaon (IN); Manoj Kumar Verma, Gurgaon (IN); Harinder Singh, Gurgaon (IN); Tanyya Khanna, Gurgaon (IN); Puja Kumari, Gurgaon (IN); and Sameer Maheshwari, Gurgaon (IN)
Assigned to BRIGHT LIFECARE PVT. LTD, Gurgaon (IN)
Filed by BRIGHT LIFECARE PVT. LTD, Gurgaon (IN)
Filed on Jan. 15, 2025, as Appl. No. 19/022,162.
Application 19/022,162 is a division of application No. 17/425,582, granted, now 12,220,447, previously published as PCT/IN2019/050865, filed on Nov. 26, 2019.
Claims priority of application No. 201911004951 (IN), filed on Feb. 7, 2019; and application No. 201911004951 (IN), filed on Oct. 25, 2019.
Prior Publication US 2025/0152678 A1, May 15, 2025
Int. Cl. A61K 38/46 (2006.01); A23L 29/00 (2016.01); A23L 33/195 (2016.01); A61K 38/47 (2006.01); A61K 38/48 (2006.01); C12N 9/50 (2006.01); C12N 9/54 (2006.01); C12N 9/58 (2006.01)

CPC A61K 38/465 (2013.01) [A23L 29/06 (2016.08); A23L 33/195 (2016.08); A61K 38/47 (2013.01); A61K 38/4826 (2013.01); A61K 38/488 (2013.01); C12N 9/54 (2013.01); C12N 9/58 (2013.01); C12N 9/63 (2013.01); C12Y 304/22002 (2013.01); C12Y 304/22004 (2013.01)]

6 Claims

1. A method of consuming a protein supplement by a human comprising said human consuming the protein supplement in combination with a proteolytic enzyme composition, wherein the proteolytic enzyme composition comprises: an acid protease, an alkaline protease, plant proteases papain and bromelain;

wherein:

weight ratio of acid protease to alkaline protease in the composition ranges from about 1:0.5 to about 1:6;

weight ratio of acid protease to plant proteases ranges from about 1:0.1 to about 1:2;

weight ratio of alkaline protease to plant proteases ranges from about 1:0.1 to about 1:2; and ratio of papain to bromelain in the composition ranges from about 1:0.5 to about 1:4;

wherein the method increases the bioavailability of each of branched amino acids isoleucine, leucine and valine by at least 50% over a control group.