	US 12,472,212 B2
	Lactiplantibacillus plantarum GOLDGUT-LP618 having function of resisting salmonella infection and application thereof
Silu Zhang, Shenzhen (CN); Yanyi Zheng, Shenzhen (CN); Guoxun Xiao, Shenzhen (CN); Xiaoli Zhou, Shenzhen (CN); Caijuan Lan, Shenzhen (CN); Yuebiao Feng, Shenzhen (CN); Tengxun Zhang, Shenzhen (CN); Xin Teng, Shenzhen (CN); and Song Huang, Shenzhen (CN)
Assigned to Shenzhen Porshealth Bioengineering Co., Ltd., Shenzhen (CN)
Filed by Shenzhen Porshealth Bioengineering Co., Ltd., Shenzhen (CN)
Filed on Nov. 6, 2024, as Appl. No. 18/938,752.
Claims priority of application No. 202311477561.6 (CN), filed on Nov. 7, 2023.
Prior Publication US 2025/0073282 A1, Mar. 6, 2025
Int. Cl. A61K 35/742 (2015.01); A61P 31/04 (2006.01); C12N 1/20 (2006.01); C12R 1/25 (2006.01)

CPC A61K 35/742 (2013.01) [A61P 31/04 (2018.01); C12N 1/205 (2021.05); C12R 2001/25 (2021.05)]

1 Claim

1. A method of preparing a microbial preparation for resisting Salmonella infection, wherein the microbial preparation comprising a Lactiplantibacillus plantarum GOLDGUT-LP618 strain, which was deposited at the China General Microbiological Culture Collection Center (CGMCC), with a deposition number of CGMCC No. 28399;

wherein the method comprising steps:

(a) inoculating the Lactiplantibacillus plantarum GOLDGUT-LP618 strain into an MRS liquid medium to form a mixture;

(b) incubating the mixture at 37° C. to obtain an enriched solution;

(c) performing a tenfold gradient on the enrichment solution to generate dilutions with gradient concentrations of 10⁻⁵, 10⁻⁶, and 10⁻⁷;

(d) applying 100 μL of each of the dilutions to sterile a first MRS solid medium and incubating under aerobic conditions at 37° C. for 24 to 48 hours, then single colonies are formed;

(e) selecting the single colonies; and

(f) repeatedly streaking the selected colonies on a second MRS solid medium for purification to obtain a purified strain.