| CPC G16H 20/30 (2018.01) [A61B 5/0075 (2013.01); A61B 5/0816 (2013.01); A61B 5/082 (2013.01); A61B 5/0836 (2013.01); A61B 5/1032 (2013.01); A61B 5/486 (2013.01); A61B 5/7415 (2013.01); A61M 21/02 (2013.01); G01N 21/78 (2013.01); G01N 21/783 (2013.01); G01N 33/84 (2013.01); G16H 20/40 (2018.01); G16H 40/40 (2018.01); G16H 40/63 (2018.01); G16H 40/67 (2018.01); G16H 50/20 (2018.01); A61B 5/097 (2013.01); A61B 5/4836 (2013.01); A61B 2560/0223 (2013.01); A61M 16/0051 (2013.01); A61M 16/024 (2017.08); A61M 2021/0027 (2013.01); A61M 2021/0088 (2013.01); A61M 2205/3303 (2013.01); A61M 2205/581 (2013.01); A61M 2205/582 (2013.01); A61M 2230/432 (2013.01); G01N 21/3151 (2013.01); G01N 2021/7773 (2013.01); G01N 2201/0231 (2013.01); G01N 2201/062 (2013.01); G01N 2201/1211 (2013.01); H04M 1/72412 (2021.01)] | 10 Claims |

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1. A method for treating a user with PTSD, the method comprising:
measuring a user's baseline end-tidal CO2 level and breathing rate using a sample tube assembly and sensing unit optically coupled via a light guide to a quantitative colorimetric carbon dioxide system comprising a temperature-controlled colorimetric indicator configured to reflect light;
controlling, via a temperature controller, a temperature of the temperature-controlled colorimetric indicator to a pre-determined temperature;
generating a measurement signal pursuant to detecting the reflected light;
measuring the user's end-tidal CO2 level and breathing rate based on the measurement signal;
providing a target end-tidal CO2 level and a target breathing rate for the user;
outputting a set of tone patterns with instructions to modify the user's end-tidal CO2 level and breathing rate from an audio device during a first time period and discontinuing the output of the set of tone patterns during a second time period, wherein the tone patterns are configured to guide a breathing pattern of the user to achieve the target end-tidal CO2 level and target breathing rate; and
instructing the user to use the tone patterns during the first time period to guide the user's breathing pattern to achieve the target end-tidal CO2 level and target breathing rate and to maintain the user's breathing pattern to achieve the target end-tidal CO2 level and target breathing rate without instruction or cues during the second time period, thereby treating the user's PTSD.
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