US 12,140,596 B2
Kits, microfluidics devices, and methods for performing biotin assays
Tie Q Wei, Wilmington, DE (US); Zhu Teng, Garnet Valley, PA (US); Xiaodong Liu, Wilmington, DE (US); and Martin Drinan, Newark, DE (US)
Assigned to Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Appl. No. 17/250,173
Filed by Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
PCT Filed Jul. 24, 2019, PCT No. PCT/US2019/043195
§ 371(c)(1), (2) Date Dec. 8, 2020,
PCT Pub. No. WO2020/028110, PCT Pub. Date Feb. 6, 2020.
Claims priority of provisional application 62/711,694, filed on Jul. 30, 2018.
Prior Publication US 2021/0247398 A1, Aug. 12, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/58 (2006.01); B01L 3/00 (2006.01); G01N 21/76 (2006.01)
CPC G01N 33/582 (2013.01) [B01L 3/502715 (2013.01); G01N 21/76 (2013.01); B01L 2200/027 (2013.01); B01L 2200/16 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A kit containing a chemiluminescent detection system for determining the concentration of biotin in a sample, the kit comprising:
(a) a composition comprising a particle having a singlet oxygen-activatable chemiluminescent compound coupled thereto and/or disposed therein, the particle having biotin or an analog thereof directly or indirectly bound thereto, wherein the singlet oxygen-activatable chemiluminescent compound is a substance that undergoes a chemical reaction with singlet oxygen to form a metastabile intermediate species that can decompose with the simultaneous or subsequent emission of light; and
(b) a composition comprising a particle having a sensitizer capable of generating singlet oxygen in its excited state coupled thereto and/or disposed therein, the particle having traptavidin directly or indirectly bound thereto; and
wherein the kit is capable of detecting biotin concentrations in a range of from about 0 to about 1500 ng/ml;
wherein the biotin analog is selected from the group consisting of 4′-hydroxyazobenzene-2-carboxylic acid (HABA), iminobiotin, biotin carbonate, biotin carbamate, biotin methyl ester, desthiobiotin, diaminobiotin, chloroacetylated biotin, biotin sulfone, thiobiotin, methoxycarbonylbiotin methyl ester, bis-biotin, tetra-biotin, and an ester or salt of any of the above biotin analogs.