US 12,140,590 B2
Compositions and methods for molecular labeling
Michael L. Samuels, Windham, NH (US); Jeffrey Charles Olson, Chelmsford, MA (US); Andrew Watson, Bedford, MA (US); Keith Brown, Carlsbad, CA (US); and Darren R. Link, Lexington, MA (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on Nov. 17, 2021, as Appl. No. 17/529,082.
Application 17/529,082 is a continuation of application No. 16/928,924, filed on Jul. 14, 2020, granted, now 11,747,327.
Application 16/928,924 is a continuation of application No. 16/795,467, filed on Feb. 19, 2020, granted, now 11,768,198.
Application 16/795,467 is a continuation of application No. 16/400,858, filed on May 1, 2019, granted, now 11,168,353.
Application 16/400,858 is a continuation of application No. 14/995,744, filed on Jan. 14, 2016, granted, now 10,584,332.
Application 14/995,744 is a continuation of application No. 14/874,553, filed on Oct. 5, 2015, granted, now 11,965,877.
Application 14/874,553 is a continuation of application No. 13/398,677, filed on Feb. 16, 2012, granted, now 9,150,852, issued on Oct. 6, 2015.
Claims priority of provisional application 61/476,714, filed on Apr. 18, 2011.
Claims priority of provisional application 61/444,612, filed on Feb. 18, 2011.
Prior Publication US 2022/0064711 A1, Mar. 3, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/53 (2006.01); C12N 15/10 (2006.01); C12Q 1/6804 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6874 (2018.01); C40B 50/08 (2006.01); G01N 33/532 (2006.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/53 (2013.01) [C12N 15/1075 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6874 (2013.01); C40B 50/08 (2013.01); G01N 33/532 (2013.01); G01N 33/5436 (2013.01); G01N 33/58 (2013.01); C12Q 2521/10 (2013.01); C12Q 2563/179 (2013.01); C12Q 2565/30 (2013.01); G01N 2458/10 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for detecting and identifying target material in a fluid sample, the method comprising:
incubating a fluid sample containing a plurality of single cells comprising target material with a plurality of antibodies conjugated to a respective plurality of target barcodes to allow the antibodies to bind to the target material;
washing away unbound antibodies;
isolating the single cells and cell barcodes within aqueous partitions;
combining the target barcodes with the cell barcodes to form composite barcodes;
releasing the composite barcodes from the antibodies; and
sequencing, after the releasing step, the composite barcodes to determine sequence identity and counts of the composite barcodes, thereby quantifying the target material of the single cells.