US 12,140,563 B2
Formation of layers of amphiphilic molecules
Stuart William Reid, Oxford (GB); Terence Alan Reid, Kidlington (GB); James Anthony Clarke, Oxford (GB); Steven Paul White, Oxford (GB); and Gurdial Singh Sanghera, Oxford (GB)
Assigned to Oxford Nanopore Technologies PLC, Oxford (GB)
Filed by Oxford Nanopore Technologies PLC, Oxford (GB)
Filed on Feb. 26, 2018, as Appl. No. 15/905,440.
Application 15/905,440 is a continuation of application No. 14/788,120, filed on Jun. 30, 2015, granted, now 9,927,398.
Application 14/788,120 is a continuation of application No. 12/339,956, filed on Dec. 19, 2008, abandoned.
Claims priority of provisional application 61/080,492, filed on Jul. 14, 2008.
Claims priority of application No. 0724736 (GB), filed on Dec. 19, 2007.
Prior Publication US 2018/0321188 A1, Nov. 8, 2018
Int. Cl. G01N 27/447 (2006.01); B01L 3/00 (2006.01); C12Q 1/6869 (2018.01); G01N 27/327 (2006.01); G01N 27/453 (2006.01); G01N 33/487 (2006.01)
CPC G01N 27/44791 (2013.01) [B01L 3/502707 (2013.01); B01L 3/50273 (2013.01); C12Q 1/6869 (2013.01); G01N 27/3278 (2013.01); G01N 27/453 (2013.01); G01N 33/48721 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/161 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0427 (2013.01)] 36 Claims
OG exemplary drawing
 
1. An apparatus for supporting a layer separating two volumes of aqueous solution, the apparatus comprising:
elements defining a chamber, the elements including a body of non-conductive material having formed therein a plurality of recesses opening into the chamber, wherein the plurality of recesses is capable of being filled by flowing aqueous solution across the body, wherein the chamber is above the plurality of recesses;
an electrode contained in each recess;
a further electrode in the chamber and outside the plurality of recesses;
a volume of aqueous solution in the plurality of recesses; and
a layer of amphiphilic molecules, extending across the opening of the plurality of recesses, separating the volume of aqueous solution in the plurality of recesses from an aqueous solution comprising amphiphilic molecules that covers the plurality of recesses;
wherein the electrode contained in each recess resides in a base of the recess;
wherein the body has a plurality of conductive paths, each conductive path extending from the electrode in a respective recess through the body and perpendicular to the base of the respective recess, configured for connection to an electrical circuit;
wherein each electrode is laterally separated from one another and there is a single electrode in each recess;
wherein each conductive path has a uniform cross-sectional dimension and has a cross-sectional dimension less than that of each electrode; and
wherein the conductive paths are attached to a low-capacitance multi-layer substrate.