US 12,140,547 B2
Digital surface-enhanced Raman spectroscopy sensing platform
Eun-Ah You, Daejeon (KR); Zhou Wei, Blacksburg, VA (US); Wonil Nam, Busan (KR); and Wansun Kim, Namyangju-si (KR)
Assigned to KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE, Daejeon (KR); and VIRGINIA TECH INTELLECTUAL PROPERTIES, INC., Blacksburg, VA (US)
Filed by KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE, Daejeon (KR); and VIRGINIA TECH INTELLECTUAL PROPERTIES, INC., Blacksburg, VA (US)
Filed on Jan. 28, 2022, as Appl. No. 17/587,541.
Claims priority of application No. 10-2021-0167646 (KR), filed on Nov. 29, 2021.
Prior Publication US 2023/0168201 A1, Jun. 1, 2023
Int. Cl. G01N 21/65 (2006.01); B82Y 35/00 (2011.01); B82Y 40/00 (2011.01); G01N 33/68 (2006.01)
CPC G01N 21/658 (2013.01) [G01N 33/6896 (2013.01); B82Y 35/00 (2013.01); B82Y 40/00 (2013.01); G01N 2021/656 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method of detecting catecholamine included in an analyte comprising:
preparing a nano-laminate in which a plasmonic metal layer and an etching layer are alternately stacked, and a surface of the nanolaminate being modified with a compound that binds to a first functional group of catecholamine, and the nanolaminate comprises nanogap formed by etching the etching layer;
preparing a metal nanoparticle whose surface is modified with a compound that binds to a second functional group of the catecholamine;
incubating the nanolaminate, the metal nanoparticle, and catecholamine;
detecting a signal through Surface-enhanced Raman spectroscopy (SERS);
wherein the average diameter of the metal nanoparticles is smaller than the width of the nanogap, and the metal nanoparticles are attracted to the nanogap, thereby generating overlapping hot spots.