US 12,140,540 B2
Stimulated Raman coupled fluorescence spectroscopy and microscopy system and method
Wei Min, Fort Lee, NJ (US); Lixue Shi, Zhejiang (CN); Hanqing Xiong, Hubei (CN); and Lu Wei, Anhui (CN)
Assigned to The Trustees of Columbia University in the City of New York, New York, NY (US)
Filed by The Trustees of Columbia University in the City of New York, New York, NY (US)
Filed on Sep. 13, 2021, as Appl. No. 17/473,259.
Application 17/473,259 is a continuation of application No. PCT/US2020/022333, filed on Mar. 12, 2020.
Claims priority of provisional application 62/817,327, filed on Mar. 12, 2019.
Prior Publication US 2021/0404958 A1, Dec. 30, 2021
Int. Cl. G01N 21/63 (2006.01); G01N 21/64 (2006.01); G01N 21/65 (2006.01)
CPC G01N 21/636 (2013.01) [G01N 21/6428 (2013.01); G01N 21/6458 (2013.01); G01N 21/65 (2013.01); G01N 2021/6439 (2013.01); G01N 2021/655 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for providing an excited fluorescence radiation, comprising:
with a beam of a probe, exciting a fluorescent compound to an excited state for a fluorescence emission to effectuate the excited fluorescence radiation comprising exciting the fluorescent compound to an intermediate vibrational state by electronic pre-resonance stimulated Raman scattering (“epr-SRS”), and then exciting the fluorescent compound in the intermediate vibrational state to the excited state; and coupling a Raman transition thereof to electronic excited-state population for subsequent fluorescence detection via stimulated Raman excited fluorescence or via stimulated Raman attenuated fluorescence,
detecting the fluorescent compound molecule-based on the fluorescence emission.