US 12,140,526 B2
Methods for detecting particles present in a cell composition
Brittney Jo Livingston, Kirkland, WA (US); and Hai Yue, Seattle, WA (US)
Assigned to Juno Therapeutics, Inc., Seattle, WA (US)
Appl. No. 16/970,917
Filed by Juno Therapeutics, Inc., Seattle, WA (US)
PCT Filed Feb. 28, 2019, PCT No. PCT/US2019/020139
§ 371(c)(1), (2) Date Aug. 18, 2020,
PCT Pub. No. WO2019/169194, PCT Pub. Date Sep. 6, 2019.
Claims priority of provisional application 62/636,840, filed on Feb. 28, 2018.
Prior Publication US 2020/0393355 A1, Dec. 17, 2020
Int. Cl. G01N 15/14 (2024.01); G01N 33/543 (2006.01); G01N 15/00 (2006.01)
CPC G01N 15/1456 (2013.01) [G01N 33/54313 (2013.01); G01N 2015/0053 (2013.01)] 36 Claims
OG exemplary drawing
 
1. A method for detecting the presence or absence of beads in a cell composition, the method comprising the steps of:
a) incubating a sample comprising at least a portion of an input composition or a sample derived from the input composition, wherein said input composition comprises one or more cells and is likely to comprise or may comprise one or more beads in which said one or more beads is capable of emitting an autofluorescence, wherein the incubating is under conditions to induce lysis of cells to produce an output composition; and
b) analyzing the output composition or a portion thereof by fluorescence imaging of the autofluorescence of the one or more beads and by bright field imaging, to obtain a fluorescent image and a brightfield image and determine the presence or absence of the one or more beads in the sample.