US 12,139,817 B2
Locus specific amplification using array probes
Glenn Fu, Dublin, CA (US); Michael Shapero, Campbell, CA (US); and Pei-Hua Wang, Fremont, CA (US)
Assigned to AFFYMETRIX, INC., Santa Clara, CA (US)
Filed by AFFYMETRIX, INC., Carlsbad, CA (US)
Filed on Aug. 5, 2022, as Appl. No. 17/817,678.
Application 17/817,678 is a division of application No. 16/402,539, filed on May 3, 2019, granted, now 11,408,094.
Application 16/402,539 is a division of application No. 15/184,393, filed on Jun. 16, 2016, granted, now 10,329,600, issued on Jun. 25, 2019.
Application 15/184,393 is a continuation of application No. 12/211,100, filed on Sep. 15, 2008, granted, now 9,388,457, issued on Jul. 12, 2016.
Claims priority of provisional application 60/972,548, filed on Sep. 14, 2007.
Claims priority of provisional application 60/972,410, filed on Sep. 14, 2007.
Prior Publication US 2022/0372654 A1, Nov. 24, 2022
Int. Cl. C40B 50/06 (2006.01); C12Q 1/6837 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6858 (2018.01)
CPC C40B 50/06 (2013.01) [C12Q 1/6837 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6858 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for amplifying a plurality of targets, the method comprising:
(a) hybridizing each target to a first complementary support bound probe in an array comprising a plurality of support bound probes, where probes of the same sequence are present in the same feature and wherein different targets hybridize to different first probes in different features;
(b) extending the first probes using the hybridized targets as template to obtain extended first probes;
(c) removing the target;
(d) ligating an adapter to 3′ end of the extended first probes, wherein the adapter comprises a top strand that is attached to the extended first probes, and a bottom strand that is hybridized to the first strand, wherein the adapter comprises an RNA polymerase promoter;
(e) transcribing multiple RNA copies of the extended first probe using RNA polymerase;
(f) allowing the copies of the extended first probe to hybridize to probes of the array in the same feature; and
(g) analyzing the copies to determine the sequence of the targets.