US 12,139,815 B2
Methods for rapid detection of immunogenic epitopes in biothreat agents and emerging pathogens for peptide vaccines and therapeutics
Magdalena Franco, Livermore, CA (US); Nicole Collette, Livermore, CA (US); and Patrik D'Haeseleer, Livermore, CA (US)
Assigned to Lawrence Livermore National Security, LLC, Livermore, CA (US)
Filed by Lawrence Livermore National Security, LLC, Livermore, CA (US)
Filed on Jan. 27, 2022, as Appl. No. 17/586,687.
Claims priority of provisional application 63/142,870, filed on Jan. 28, 2021.
Prior Publication US 2022/0238183 A1, Jul. 28, 2022
Int. Cl. C40B 30/04 (2006.01); C40B 40/10 (2006.01); G16B 20/30 (2019.01); G16B 40/20 (2019.01)
CPC C40B 30/04 (2013.01) [C40B 40/10 (2013.01); G16B 20/30 (2019.02); G16B 40/20 (2019.02)] 11 Claims
OG exemplary drawing
 
1. A method of identifying an immunogenic peptide comprising:
a) identifying an interactive peptide by contacting a peptide library generated from a pathogenic agent with purified peptide-specific immunoglobulins under conditions to form peptide-immunoglobulin complexes between the interactive peptides and the specific immunoglobulins and identifying one or more interactive peptides bound to the peptide-immunoglobulin complexes; and
b) comparing the identified interactive peptides of step (a) to peptides bound to control immunoglobulins, and selecting at least one identified peptide of step (a) that does not bind to the control immunoglobulins or is enriched in the interactive peptides of step (a) as compared to the peptide bound to control immunoglobulins, thereby identifying an immunogenic peptide.