	US 12,139,770 B2
	Methods and assays for male sterile watermelon
Eleni Bachlava, Fairfield, CA (US); Tarek Joobeur, Sacramento, CA (US); and Gregory E. Tolla, Woodland, CA (US)
Assigned to SEMINIS VEGETABLE SEEDS, INC., St. Louis, MO (US)
Filed by Seminis Vegetable Seeds, Inc., St. Louis, MO (US)
Filed on Jul. 13, 2020, as Appl. No. 16/927,890.
Application 16/376,965 is a division of application No. 14/733,767, filed on Jun. 8, 2015, granted, now 10,316,369, issued on Jun. 11, 2019.
Application 16/927,890 is a continuation of application No. 16/376,965, filed on Apr. 5, 2019, granted, now 10,801,075.
Claims priority of provisional application 62/018,413, filed on Jun. 27, 2014.
Prior Publication US 2021/0047700 A1, Feb. 18, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A01H 1/04 (2006.01); A01H 5/08 (2018.01); C12N 15/82 (2006.01); C12Q 1/6895 (2018.01)

CPC C12Q 1/6895 (2013.01) [A01H 1/04 (2013.01); A01H 5/08 (2013.01); C12N 15/8289 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)]

12 Claims

1. A method of detecting in at least one watermelon plant or part thereof a genotype indicative of a male sterile phenotype, the method comprising the steps of:

obtaining a sample of nucleic acids from at least one watermelon plant or part thereof; and

detecting in said sample the presence of an allele of at least a first polymorphic nucleic acid marker in a genomic region on chromosome 6, wherein said genomic region comprises a locus that confers said male sterile phenotype, and wherein said genomic region is flanked by and comprises marker locus NW0248249 having SEQ ID NO: 22 and marker locus NCLAN009405170 having SEQ ID NO: 59, thereby detecting said genotype.