	US 12,139,768 B2
	Polymerase chain reaction primers and probes for Mycobacterium tuberculosis
David Alland, Bernardsville, NJ (US); and Soumitesh Chakravorty, Somerset, NJ (US)
Assigned to RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY, New Brunswick, NJ (US)
Filed by Rutgers, The State University of New Jersey, New Brunswick, NJ (US)
Filed on Nov. 16, 2021, as Appl. No. 17/527,576.
Application 17/527,576 is a continuation of application No. 15/517,042, granted, now 11,180,816, previously published as PCT/US2015/054916, filed on Oct. 9, 2015.
Claims priority of provisional application 62/062,351, filed on Oct. 10, 2014.
Prior Publication US 2022/0064715 A1, Mar. 3, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/689 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6865 (2018.01); G01N 33/50 (2006.01)

CPC C12Q 1/689 (2013.01) [C12Q 1/686 (2013.01); C12Q 1/6865 (2013.01); G01N 33/5008 (2013.01); C12Q 2525/143 (2013.01); C12Q 2525/185 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01)]

11 Claims

1. An isolated nucleic acid probe for detecting drug-resistant M.tb comprising a probe sequence that is at least 85% identical to SEQ ID No: 56 in its entirety, wherein the probe is 38 to 50 nucleotides in length, and wherein the probe is labeled with a fluorophore and a quencher at its two ends respectively.