US 12,139,748 B2
Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
Colin Ng, Branford, CT (US); Alaina Kaiser, West Haven, CT (US); Emily Bettini, Waterbury, CT (US); Patrick Paczkowski, East Haven, CT (US); Sean MacKay, New Haven, CT (US); Feimei Liu, Branford, CT (US); and Maithreyan Srinivasan, Branford, CT (US)
Assigned to IsoPlexis Corporation, Branford, CT (US)
Filed by IsoPlexis Corporation, Branford, CT (US)
Filed on Jun. 10, 2022, as Appl. No. 17/837,668.
Application 17/837,668 is a continuation of application No. 16/349,183, granted, now 11,493,508, previously published as PCT/US2017/061344, filed on Nov. 13, 2017.
Claims priority of provisional application 62/544,521, filed on Aug. 11, 2017.
Claims priority of provisional application 62/421,031, filed on Nov. 11, 2016.
Prior Publication US 2022/0390446 A1, Dec. 8, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6813 (2018.01); C12Q 1/6806 (2018.01); G01N 33/531 (2006.01); G01N 33/68 (2006.01)
CPC C12Q 1/6813 (2013.01) [C12Q 1/6806 (2013.01); G01N 33/531 (2013.01); G01N 33/6842 (2013.01)] 20 Claims
 
1. A device for multiplexed analysis of intracellular targets of a single cell, the device comprising:
a surface comprising a plurality of antibodies operatively-linked thereto, wherein:
each antibody is configured to specifically bind to a distinct intracellular target, and
the plurality of antibodies form a repeating pattern;
a substrate comprising a plurality of chambers, the plurality of chambers each comprising a biocompatible coating, a size exclusion well configured to accommodate a single bead, and a bead disposed within the size exclusion well, wherein:
the substrate is configured to releasably couple with the surface, such that, each chamber of the plurality of chambers is aligned and in fluid communication with at least one repeat of the repeating pattern of the plurality of antibodies of the surface, and wherein the bead is coupled to an oligonucleotide comprising a barcode sequence and a sequence configured to specifically bind to a nucleic acid sequence of a cell.