US 12,139,745 B2
Processing particle-containing samples
Kalyan Handique, Ypsilanti, MI (US); Gene Parunak, Saline, MI (US); Aaron Kehrer, Ypsilanti, MI (US); Betty Wu, Canton, MI (US); and Karthik Ganesan, Ann Arbor, MI (US)
Assigned to HandyLab, Inc., Franklin Lakes, NJ (US)
Filed by HandyLab, Inc., Franklin Lakes, NJ (US)
Filed on Jul. 29, 2021, as Appl. No. 17/388,785.
Application 17/388,785 is a continuation of application No. 17/113,853, filed on Dec. 7, 2020, granted, now 11,078,523.
Application 17/113,853 is a continuation of application No. 16/911,065, filed on Jun. 24, 2020, granted, now 10,865,437, issued on Dec. 15, 2020.
Application 16/911,065 is a continuation of application No. 15/612,105, filed on Jun. 2, 2017, granted, now 10,731,201, issued on Aug. 4, 2020.
Application 15/612,105 is a continuation of application No. 14/223,829, filed on Mar. 24, 2014, granted, now 9,670,528, issued on Jun. 6, 2017.
Application 14/223,829 is a continuation of application No. 12/702,648, filed on Feb. 9, 2010, granted, now 8,679,831, issued on Mar. 25, 2014.
Application 12/702,648 is a continuation of application No. 10/567,002, granted, now 7,731,906, issued on Jun. 8, 2010, previously published as PCT/US2004/025181, filed on Aug. 2, 2004.
Claims priority of provisional application 60/553,553, filed on Mar. 17, 2004.
Claims priority of provisional application 60/551,785, filed on Mar. 11, 2004.
Claims priority of provisional application 60/491,269, filed on Jul. 31, 2003.
Prior Publication US 2022/0010364 A1, Jan. 13, 2022
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6806 (2018.01); G01N 1/40 (2006.01); B33Y 80/00 (2015.01)
CPC C12Q 1/6806 (2013.01) [B01L 3/502707 (2013.01); B01L 3/502738 (2013.01); B01L 3/502753 (2013.01); G01N 1/40 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0887 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0677 (2013.01); B33Y 80/00 (2014.12); C12Q 2523/109 (2013.01); Y10T 436/25 (2015.01); Y10T 436/25375 (2015.01)] 20 Claims
 
1. A system comprising:
a microfluidic device comprising:
a first pathway comprising:
a first port configured to receive a polynucleotide-containing sample from a sample input device;
a region configured to receive the polynucleotide-containing sample from the first port;
a waste chamber, wherein excess fluid is configured to exit the region along an exit path to the waste chamber, wherein polynucleotides from the polynucleotide-containing sample are configured to be retained within the region as the excess fluid passes into the waste chamber;
a first channel connecting the first port and the region;
wherein the first pathway comprises an s-shaped channel; and
a second pathway comprising:
a second port;
a reaction chamber;
a second channel connecting the second port and the reaction chamber;
a heat source located external to the reaction chamber in a separate component from the microfluidic device that comprises a receptacle that receives the microfluidic device, wherein the heat source is configured to direct heat to the reaction chamber to amplify polynucleotides from the polynucleotide-containing sample within the reaction chamber when the polynucleotides are received in the reaction chamber;
a fluorescence detector configured to detect the presence of the polynucleotides within the reaction chamber when the polynucleotides are received in the reaction chamber;
a first valve configured to be actuated, the first valve comprising a state that allows passage of material along the first channel, the first valve comprising a state that obstructs passage of material along the first channel; and
a second valve configured to be actuated, the second valve comprising a state that allows passage of material along the second channel, the second valve comprising a state that obstructs passage of material along the second channel.