US 12,139,737 B2
Host cells comprising a recombinant casein protein and a recombinant kinase protein
Viviane Lanquar, San Carlos, CA (US); Magi El-Richani, San Francisco, CA (US); Leyla Hathwaik, South San Francisco, CA (US); and Yaxin Wang, South San Francisco, CA (US)
Assigned to Nobell Foods, Inc., South San Francisco, CA (US)
Filed by Nobell Foods, Inc., South San Francisco, CA (US)
Filed on Sep. 8, 2023, as Appl. No. 18/463,864.
Application 18/463,864 is a continuation of application No. 18/064,199, filed on Dec. 9, 2022, granted, now 11,840,717.
Application 18/064,199 is a continuation of application No. PCT/US2021/053002, filed on Sep. 30, 2021.
Claims priority of provisional application 63/240,621, filed on Sep. 3, 2021.
Claims priority of provisional application 63/221,642, filed on Jul. 14, 2021.
Claims priority of provisional application 63/189,547, filed on May 17, 2021.
Claims priority of provisional application 63/174,244, filed on Apr. 13, 2021.
Claims priority of provisional application 63/152,694, filed on Feb. 23, 2021.
Claims priority of provisional application 63/138,089, filed on Jan. 15, 2021.
Claims priority of provisional application 63/129,720, filed on Dec. 23, 2020.
Claims priority of provisional application 63/121,468, filed on Dec. 4, 2020.
Claims priority of provisional application 63/116,528, filed on Nov. 20, 2020.
Claims priority of provisional application 63/085,899, filed on Sep. 30, 2020.
Prior Publication US 2024/0043900 A1, Feb. 8, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 21/06 (2006.01); A23C 20/00 (2006.01); A23J 3/10 (2006.01); C07K 14/47 (2006.01); C12N 9/64 (2006.01)
CPC C12P 21/06 (2013.01) [A23C 20/00 (2013.01); A23J 3/10 (2013.01); C07K 14/4732 (2013.01); C12N 9/6424 (2013.01); C12N 9/6483 (2013.01); C12Y 304/21009 (2013.01); C07K 2319/00 (2013.01)] 26 Claims
 
1. A plant cell comprising:
a) a heterologous casein protein; and
b) a heterologous Fam20C kinase protein;
wherein the heterologous casein protein is intracellularly phosphorylated by the Fam20C kinase protein: wherein the plant cell accumulates higher quantities of the heterologous casein protein than a control plant cell lacking the heterologous Fam20C kinase protein.