US 12,139,725 B2
Composition for promoting stem cell differentiation, comprising progenitor cell culture solution and multilayer graphene film, and use thereof
Jeong Mook Lim, Seoul (KR); Ji Yeon Ahn, Seoul (KR); Jong Bo Park, Seoul (KR); Byung Hee Hong, Suwon-Si (KR); and Woo Sub Yang, Seoul (KR)
Assigned to SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR); and BIOGRAPHENE INC., Suwon-si (KR)
Appl. No. 17/055,441
Filed by SEOUL NATIONAL UNIVERSITY R & DB FOUNDATION, Seoul (KR); and BIOGRAPHENE INC., Suwon-si (KR)
PCT Filed May 14, 2019, PCT No. PCT/KR2019/005765
§ 371(c)(1), (2) Date Nov. 13, 2020,
PCT Pub. No. WO2019/221477, PCT Pub. Date Nov. 21, 2019.
Claims priority of application No. 10-2018-0055015 (KR), filed on May 14, 2018.
Prior Publication US 2021/0180018 A1, Jun. 17, 2021
Int. Cl. C12N 5/077 (2010.01); A61K 35/32 (2015.01); C01B 32/186 (2017.01)
CPC C12N 5/0654 (2013.01) [A61K 35/32 (2013.01); C01B 32/186 (2017.08); C12N 2506/1384 (2013.01)] 8 Claims
 
1. A method comprising culturing stem cells in a composition comprising an osteochondroprogenitor cell culture solution and a multilayer graphene film as active ingredients, and thereby promoting the growth and osteogenic differentiation of stem cells,
wherein the multilayer graphene film has 2 to 7 layers formed by lamination of single-layered graphene.