US 12,139,716 B2
Composition for editing a nucleic acid sequence and method using the same
Lung-Jr Lin, Taichung (TW); and Yi-hua Hsu, Taipei (TW)
Appl. No. 16/472,274
Filed by Lung-Jr Lin, Taichung (TW); and Yi-hua Hsu, Taipei (TW)
PCT Filed Dec. 20, 2017, PCT No. PCT/US2017/067484
§ 371(c)(1), (2) Date Jun. 21, 2019,
PCT Pub. No. WO2018/119021, PCT Pub. Date Jun. 28, 2018.
Claims priority of provisional application 62/437,347, filed on Dec. 21, 2016.
Prior Publication US 2019/0323019 A1, Oct. 24, 2019
Int. Cl. C12N 15/73 (2006.01); C12N 15/66 (2006.01); C12N 15/79 (2006.01); C12N 15/90 (2006.01)
CPC C12N 15/73 (2013.01) [C12N 15/66 (2013.01); C12N 15/79 (2013.01); C12N 15/907 (2013.01)] 16 Claims
 
1. A composition for editing a target nucleic acid sequence, comprising:
one or more nucleic acid molecules each comprising an artificial nucleic acid sequence flanked with capping sequences, wherein each of the capping sequences is homologous to a region in the target nucleic acid sequence, wherein the nucleic acid molecule is a double-stranded DNA, and wherein the artificial nucleic acid sequence is an intron sequence comprising at least one of a splice donor site, a splice acceptor site, a branch site, a selection marker, and any combination thereof, wherein the selection marker comprises a promoter operably linked to a reporter gene to allow a selection for an integrant in genome, and wherein the artificial nucleic acid sequence is removed by RNA splicing during maturation of an RNA product of the target nucleic acid sequence; and
Lambda beta protein or a linear or circular vector comprising a nucleic acid sequence encoding the Lambda beta protein.