US 12,139,715 B2
Gene expression cassette capable of initiating translation after completion of transcription for producing high-quality recombinant protein in bacteria
Sang Woo Seo, Seoul (KR); and Jina Yang, Seoul (KR)
Assigned to SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR)
Appl. No. 17/253,401
Filed by SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR)
PCT Filed Jun. 19, 2018, PCT No. PCT/KR2018/006924
§ 371(c)(1), (2) Date Dec. 17, 2020,
PCT Pub. No. WO2019/245066, PCT Pub. Date Dec. 26, 2019.
Claims priority of application No. 10-2018-0070484 (KR), filed on Jun. 19, 2018.
Prior Publication US 2021/0340551 A1, Nov. 4, 2021
Int. Cl. C12N 15/67 (2006.01)
CPC C12N 15/67 (2013.01) [C12N 2840/55 (2013.01)] 6 Claims
 
1. A DNA molecule capable of forming a stem loop structure during transcription into mRNA, the stem loop structure having a ribosome-binding site located therein, the DNA molecule comprising:
a first DNA sequence encoding a first RNA sequence designed such that a part of the first RNA sequence can participate in a formation of the stem loop structure; and
a second DNA sequence encoding a second RNA sequence located after a stop codon that can complementarily bind to the first RNA sequence designed such that the part of the first RNA sequence can participate in the formation of the stem loop structure.